Loading presentation...

Present Remotely

Send the link below via email or IM

Copy

Present to your audience

Start remote presentation

  • Invited audience members will follow you as you navigate and present
  • People invited to a presentation do not need a Prezi account
  • This link expires 10 minutes after you close the presentation
  • A maximum of 30 users can follow your presentation
  • Learn more about this feature in our knowledge base article

Do you really want to delete this prezi?

Neither you, nor the coeditors you shared it with will be able to recover it again.

DeleteCancel

Make your likes visible on Facebook?

Connect your Facebook account to Prezi and let your likes appear on your timeline.
You can change this under Settings & Account at any time.

No, thanks

Unknown bacteria

Microbiology
by

Gabriela Martinez

on 8 December 2014

Comments (0)

Please log in to add your comment.

Report abuse

Transcript of Unknown bacteria

Microbiology W-02
By: Idalia, Luis, Lesley, & Gabby

UNKNOWN BACTERIA
*
Isolate and identify bacteria from an unknown sample.

*
exposed the sample to several means of identifying bacteria, including Gram, Acid-fast, and Endospore differential stains, as well as specialized media.

*
Our culture number 5 was randomly selected and it was subjected to qualitative tests.
Objective of the exercise
Materials
*
Microscope
*
Incubator
*
Stains

*
Reagents
*
Bacteria samples
*
Broth tubes

*
Slant tubes
*
Media plates
Techniques
*
A Gram stain was done to differentiate the unknown sample from culture #9 E. coli, and culture #20 Staphylococcus aureus.

*
The Gram stain produced satisfactory results with a clear indication of

negativity. Light pink staining was evident on the cells in the FOV(field of view) and a search of the slide revealed uniformity among the sample.
*
From this result it was concluded that culture unknown #5 was Gram

negative in nature, therefore it could possible be any of the following bacteria: Alkaligenes faecolis, E. coli, Enterobacter aerogenes, Proteus vulgaris, or Pseudomonas aeruginosa.
GRAM STAIN
GRAM STAIN RESULTS
Culture
Unknown #5
Cuture #9
E. coli
Culture #20
S. aureus
A Staphylococcus aureus is a Gram-positive bacteria that grows in grape-like clusters. Under the microscope they appear round (cocci).
Note gram-negative (pink)
bacilli.
Note gram-negative (pink) bacilli.
Effects of Osmotic Pressure and Bacteria Growth
in this pocedure we used cultures #9 E.coli, #20 staphylococcus and our unknown bacteria #5
the objective of the lab was to take this group of bacteria and watch its ability to grow in environments with different NaCl concentrations.
Results:
in this lab we concluded that E.coli and the unknown #5 were very similar.
Both cultures #9 and #5 (uk) were positive for growth in NaCl .5% and 5% and both had no growth in 15% NaCl
NaCl .5%
NaCl 5%
NaCl 15%
E.coli #9
S.aureus #20
Unknown #5
+
+
-
+
+
+
+
+
-
E.coli
UK #5
The objective in this lab is to differentiate among the different group of bacteria. In this case bacteria #9 E. coli, #1 Alkaligenes faecolis, #15 Proteus Vulgaris, UK #5.
TSI Agar
Differentiation is made on the basis of differences in carbohydrate fermentation patterns and hydrogen sulfide production.
Results:
From the results of the lab we concluded that the unknown bacterium was not # 9 E. coli. The two cultures looked completely different.
Culture #9 had a yellow slant and a yellow butt with a bubble on the slant. This means there was lactose and sucrose fermentation and gas production.
Culture #5 (UK) had a red slant and yellow butt and a small gas bubble on the slant. This mean there was glucose fermentation.
E.coli
UK #5
SIMMON’S CITRATE TEST
Blue:
Positive citrate test
Green:
negative/no growth

We used culture
#9 E.coli
#7 Entrerobacter aerogenes
Uk
Isolation and Identification of Streptococci
We used culture
#15 proteus vulgaris
#7 Entrerobacter aerogenes
Uk
Identification of Unknown #5
There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes, such as determining if the unknown could cause illnesses in living things or knowing what microorganisms are needed to make antibiotics. The experiment was done by applying methods in order to identify an unknown bacterium. E.aerogenes is commonly responbsible for infections in hospitals.
Enterotube II Results
Glucose-positive
lysine- positive
adonitol-positive
lactose-negative
arabinose-positive
sorbitol- negative
Identificication of Unknown
The End
Full transcript