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DNA Microarrays

for class
by

Sultan Şahin

on 16 March 2011

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Transcript of DNA Microarrays

Microarray application In the past,
classified diffferent types of cancer
based on organs. In the future,
based on patterns on gene activity. What's DNA Microarray technology used for? What's DNA microarray technology? All of the cells in the human body
contain identical material But the same genes are not active in every cell. Microarray technology evolved from Southern blotting, where fragmented DNA is attached to a substrate and then probed with a known gene or fragment. Figure 1. The power of genomics and proteomics: microarray applications. DNA microarray principle


Microarray production process
Target preparation
Hybridization
Slide scanning
Data analysis
Expression profile clustering
Microarray production process: glass slides (microarrays) 2400 clones by membrane
radioactive labelling
1 experimental condition by membrane
Size: 12cm x 8cm Detail high density filters(microarrays) Size: 5,4cm x 0,9cm
Detail 10000 clones by slide
fluorescent labelling
2 experimental conditions by slide oligonucleotides chips
300000 oligonucleotides by slide
fluorescent labelling
1 experimental condition by slide
Detail Size: 1,28cm x 1,28cm
Target preparation
Hybridisation
Slide scanning
Data analysis
Expression profile clustering
Applications of Microarrays


Gene discovery: identification of new genes, know about their functioning and expression levels Disease diagnosis: heart diseases, mental illness, infectious disease and especially the study of cancer. it will be possible for the researchers to further classify the types of cancer on the basis of the patterns of gene activity in the tumor cells.
Toxicological research: Microarray technology provides a robust platform for the research of the impact of toxins on the cells and their passing on to the progeny. Figure : Difference between forward phase and reverse phase protein microarrays. A: Forward phase protein microarray; B: reverse phase protein microarray.. Drug discovery: Comparative analysis of the genes from a diseased and a normal cell will help the identification of the biochemical constitution of the proteins synthesized by the diseased genes.

Microarrays evolved from Southern blotting, where fragmant DNA is attached to a substrate and then probed with a known gene or fragment
Full transcript