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Subgingival biofilms Wenckebach

Subgingival biofilms
by

Vincent Zijnge

on 4 July 2014

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Transcript of Subgingival biofilms Wenckebach

Root
Bone
gingiva
Pocket
Subgingivale biofilms
Vincent Zijnge
Epithelium
Biofilm
1
2
3
4
Outer layer
toplayer
intermediate layer
basal layer
13 Phyla
82 Genera
645 Species
All in the same pocket?
83 mg plaque
Core microbiome
Many species are detected sometimes
Some species are detected many times
24-100 species per site
Non-surgical therapy aims to:
eliminate both living bacteria in the microbial biofilm
remove calcified biofilm microorganisms from the tooth surface and adjacent soft tissues.
H37 Lindhe et al. 2008
living bacteria
17.430.000.000 bacterie cellen
Griffen et al. 2011
Zaura et al. 2009, Griffen et al. 2011.
P. gingivalis
T. forsythia
Actinomyces sp.
F. nucleatum
T. denticola
A. actinomycetemcomitans
Boutaga et al. 2005, Socransky et al. 2005, Rylev and Kilian 2008, Zijnge et al. 2010 and others.
20-30
90-100
80-90
50-70
70-100
90-100
P. intermedia
50-80
Prevalentie (%)
Verstoring van de biofilm structuur
microbial biofilm
Waarom ruimt het lichaam de bacteriën niet zelf op?
Waarom toch zo vaak succes?
Dr. J. van Dijk
Subgingivale biofilms
veel cellen
grote diversiteit
structuur/organisatie
Bedankt voor uw aandacht
Loe et al. 1965
Kolenbrander et al. 2006
Zaura-arite et al. 2001
Gram positive bacterium
DNA
Cell membrane
Cell wall
peptidoglycan
protein
RNA
Outer membrane
Gram negative bacterium
growth
beta-lactams
penicillin,
amoxicillin,
augmentin
Tetracycline,
doxycycline
Metronidazole,
Ciprofloxacin,
Clindamycin
Polymyxin
Full transcript