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Just a spider bite ?

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Eboni Johnson

on 7 April 2014

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Transcript of Just a spider bite ?

Just a spider bite ?
Antimicrobial Resistance and Susceptibility
Case # 1: Brent
Case # 2: Kristen
Symptoms :
Small abscess on his right elbow
.
pain
swelling
redness
Prescription
Oxacillin
g
Symptoms:
Knee surgery due to sports injury.
pain
fever
redness/inflammation
Prescription:
Oxacillin
Brent:
the lesion was gone
Outcomes after Medication
Kristen:
symptoms did not improve
still had high fever
increased fatigue
Part II: Identifying the Pathogen
Part I: Infections
Observation:
yellow colonies
Tryptic Soy Agar Plate Results
Observation
coagulase positive
Brent and Kristen were diagnosed with having just a nasty staph infection. Brent was cured. Kristen was not.
Why??????
Hypotheses
1. Time was factors.
2.The amount of dosage.
3.Types of bacteria.
4. How the bacteria was contracted.
Part III: DNA Analysis
MRSA
Microbiologist wanted to know if they had become infected with MRSA.
#1: DNA isolated from bacterial strain.
#2: PCR was used to create copies the segment of DNA
#3: Compare the DNA using a BLAST search to identify resistance.
BLAST Search
Results:
Kristen has MRSA (Methicillian- Resistant
Staphylococcus aureus
)
Reasoning
Kristen coded for the
mecA
gene, which recognizes
S. aureus
as MRSA
PCR and DNA sequencing did not provide results for Brent because he did not have MRSA.
Additional Information
MecA
codes for the protein PBP2a (penicillin-binding protein)
Having this BLAST database provide the foundation for biomedical research and discovery.
Part IV: Bacterial Resistance
Antibiotic Resistant
MRSA is antibiotic resistant to methicillin
The difference between the two cases was the
mecA
gene.
MecA gene is not commonly found in S. aureus.
MRSA the "Super Bug"
Resistant to a host of antibiotics.
MRSA stands for Methicillin Restitant
Staphylococcus aureus.
Methicillin was the first drug used for MRSA but was later change to Oxicillin because of harmful side effects.
Horizontal gene transfer (HGT)
Bacteria way of transfering DNA from one to another.
Conjugation is the cell directly passing from one cell to a reciept.
MRSA can "turn on" or "turn off" the ability to express the
mecA
gene.
MecR1 induced.
What is the purpose of the HGT?
In Conclusion
S. aureus is commonly found on the skin of humans and is not harmful until it enters the body. Once it has entered the body S. aureus can cause harmful infections for example MRSA. With the mecA gene S. aureus is antibiotic resistant to many antibiotics. The BLAST search can be used to locate the mecA gene. It is not a spider bite, it's MRSA, be smart and be safe!
Questions????
Work Cited
Afshar, Pejman. "Coagulase Test." Welcome to MicroBugz. N.p., n.d. Web. 6 Apr. 2014. <http://www.austincc.edu/microbugz/coagulase_test.php>.
Centers for Disease Control and Prevention. Centers for Disease Control and Prevention, 04 Mar. 2014. Web. 06 Apr. 2014
"Drug Resistance in MRSA Is Finely-tuned." Public Health. N.p., n.d. Web. 07 Apr. 2014
"MRSA." MRSA. N.p., n.d. Web. 07 Apr. 2014
"The Journal of the American Board of Family Medicine." It's Not a Spider Bite, It's Community-Acquired Methicillin-Resistant Staphylococcus Aureus. N.p., n.d. Web. 07 Apr. 2014.
"Result Filters." National Center for Biotechnology Information. U.S. National Library of Medicine, n.d. Web. 07 Apr. 2014
"Using Nucleotide." National Center for Biotechnology Information. U.S. National Library of Medicine, n.d. Web. 07 Apr. 2014.

Danielle Frederick
Kierra Washington
Eboni Johnson
Full transcript