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Just a spider bite ?
Transcript of Just a spider bite ?
Antimicrobial Resistance and Susceptibility
Case # 1: Brent
Case # 2: Kristen
Small abscess on his right elbow
Knee surgery due to sports injury.
the lesion was gone
Outcomes after Medication
symptoms did not improve
still had high fever
Part II: Identifying the Pathogen
Part I: Infections
Tryptic Soy Agar Plate Results
Brent and Kristen were diagnosed with having just a nasty staph infection. Brent was cured. Kristen was not.
1. Time was factors.
2.The amount of dosage.
3.Types of bacteria.
4. How the bacteria was contracted.
Part III: DNA Analysis
Microbiologist wanted to know if they had become infected with MRSA.
#1: DNA isolated from bacterial strain.
#2: PCR was used to create copies the segment of DNA
#3: Compare the DNA using a BLAST search to identify resistance.
Kristen has MRSA (Methicillian- Resistant
Kristen coded for the
gene, which recognizes
PCR and DNA sequencing did not provide results for Brent because he did not have MRSA.
codes for the protein PBP2a (penicillin-binding protein)
Having this BLAST database provide the foundation for biomedical research and discovery.
Part IV: Bacterial Resistance
MRSA is antibiotic resistant to methicillin
The difference between the two cases was the
MecA gene is not commonly found in S. aureus.
MRSA the "Super Bug"
Resistant to a host of antibiotics.
MRSA stands for Methicillin Restitant
Methicillin was the first drug used for MRSA but was later change to Oxicillin because of harmful side effects.
Horizontal gene transfer (HGT)
Bacteria way of transfering DNA from one to another.
Conjugation is the cell directly passing from one cell to a reciept.
MRSA can "turn on" or "turn off" the ability to express the
What is the purpose of the HGT?
S. aureus is commonly found on the skin of humans and is not harmful until it enters the body. Once it has entered the body S. aureus can cause harmful infections for example MRSA. With the mecA gene S. aureus is antibiotic resistant to many antibiotics. The BLAST search can be used to locate the mecA gene. It is not a spider bite, it's MRSA, be smart and be safe!
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