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The Affect of pH on Daphnia Heart Rate

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by

Ellen Ferguson

on 2 April 2017

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Transcript of The Affect of pH on Daphnia Heart Rate

Introduction
Background:
Methods
- Test one control group in tank water

- Count heart beat 4 times (6 secs each)

-Test pH 6, 5, 4 with same daphnia

-Repeat experiment for 3 trials

Results
The Affect of pH on Daphnia Heart Rate
Introduction
Acidification of lakes and ponds becoming more prevalent
Natural and anthropogenic causes
Hypothesis
-Decreasing pH will cause a sharp decline in heart rate

-Can not adapt to pH outside of optimal range
Equipment
- Dissecting Microscope
- 4 Petri Dishes
- Cheesecloth
- Lemon
- Knife
- Three daphnia
- pH Strips
- Dropper
- Tank Water
Photos
Objective
Predictions
Discussion
Summary
Future Work and Experiments
-Determine affect of acidic pH on heart rate
-Apply results to different ecosystems and environment
-Fastest at pH 6
-Slower at pH 5
-Very slow at pH 4
Daphnia - useful bioindicators of toxicity levels
Optimal pH range is 7-8.5
Lower pH should induce stress response
Increase in acidity
Stress
No homeostatic mechanisms
Daphnia: The Common Water Flea. 19 Feb. 2015. Science Buddies. Web. 11 Nov. 27.
P/H Scale. N.d. The P/H Scale. Web. 27 Nov. 2016.
Data opposed hypothesis
Heart rate did not decrease linearly
pH:
7 - (Control)
6 - daphnia heart rate decreased slightly
5 - sharp increase
Daphnia became fidgety
4 - heart beating irregularly, slowed
Statistical significant difference
Table 1 - Data Collected
Table 2 - Average heart beats per minute per Daphnia
Table 3 – Standard Deviation, Standard Error and 95% CI
More accurate pH indicators
Determining exact pH level where life becomes unsustainable
Testing in basic water
Could be used as bioindicators
Possibly better options
Research Project - Ellen Ferguson, Sarah Ashton
Wikimedia. "Daphnia Pulex." Category:Daphnia Pulex - Wikimedia Commons. N.p., n.d. Web. 29 Nov. 2016.
Wilson, Rebekah. "Acid Rain's Effect on Human Health - Infogram, Charts & Infographics." Create Online Charts & Infographics. N.p., 2016. Web. 29 Nov. 2016.
Nature Picture Library. "Water Fleas Photo." ARKive. N.p., n.d. Web. 29 Nov. 2016.
Works Cited:

Ebert, Dieter. "Introduction to Daphnia Biology." Pubmed, 2005, www.ncbi.nlm.nih.gov/books/NBK2042/.

New World Encyclopedia contributors. "Daphnia." New World Encyclopedia, 26 July 2013, www.newworldencyclopedia.org/entry/Daphnia#Feeding_behavior. Accessed 18 Oct. 2016.

Stollewerk, Angelika. "The water flea Daphnia - a 'new' model system for ecology and evolution?" US National Library of Medicine, 13 Jan. 2010, www.ncbi.nlm.nih.gov/pmc/articles/PMC2871515/. Accessed 18 Oct. 2016.

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