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Biotechnology Assignment: Insulin Production

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Gurrattan Chandhoke

on 10 December 2013

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Transcript of Biotechnology Assignment: Insulin Production

Insulin Production
Biotechnology Assignment
http://www.dailytech.com/Novo+Nordisk+Israeli+Startup+Race+to+Development+Insulin+Pill+Alternative+to+Injections/article33529.htm
Overview
http://www.nist.gov/oles/forensics/biology_dna.cfm
Insulin Background
Recombinant DNA Technology in E.coli Bacteria
Alternative Production of Insulin

people worldwide have diabetes
people will have diabetes by 2030
347 million
PURPOSE OF Insulin
Small, Simple Protein
Allows Glucose to Enter Body Cells
Production OF INSULIN
Produced in Pancreas
Two separate chains of amino acids
Cluster of Cells produce Insulin
Insulin BACKGROUND
http://www.stem-works.com/subjects/18-genetics
http://www.webmd.com/digestive-disorders/picture-of-the-pancreas
http://www.nobelprize.org/educational/medicine/insulin/diabetes-insulin.html
http://stemcells.nih.gov/info/scireport/pages/chapter7.aspx
Diabetes
Type 1: Autoimmune Disease
Type 2: Insulin Immunity
http://science.howstuffworks.com/life/human-biology/diabetes.htm
http://www.ihomeremedy.net/home-remedies-for-diabetes/
Replication Enzyme
Gene Replicated with e.coli bacteria
DNA LIGASE
MANUFACTURING INSULIN
Recombinant DNA Technology
Insulin Production in E.COLI Bacteria
http://www.littletree.com.au/dna.htm
Introduction to Process of Recombination DNA
http://www.youtube.com/watch?v=zlqD4UWCuwsd.

Insulin Structure
http://sphweb.bumc.bu.edu/otlt/mph-modules/ph/ph709_a_cellular_world/ph709_a_cellular_world7.html
Alternative Production of insulin
Cows and Pigs
Safflower Plants
http://www.littletree.com.au/dna.htm
http://www.littletree.com.au/dna.htm
http://www.littletree.com.au/dna.htm
http://www.littletree.com.au/dna.htm
http://www.littletree.com.au/dna.htm
http://www.littletree.com.au/dna.htm
http://www.littletree.com.au/dna.htm
http://www.littletree.com.au/dna.htm
http://www.littletree.com.au/dna.htm
http://diabeteshealth.com/read/2010/04/28/6660/insulin-producing-cells-derived-from-specially-bred-pigs/
http://www.scientificamerican.com/blog/post.cfm?id=new-zealand-company-injects-insulin-2009-07-23
http://endocrinevet.blogspot.ca/2010/11/insulin-choices-for-diabetic-cat-and.html
http://en.wikipedia.org/wiki/Safflower
http://www.worldhealth.net/news/safflower-oil-may-help-ward-heart-disease/
Conclusion
http://www.health.com/health/gallery/0,,20390441,00.html
http://www.pharmacology2000.com/Diabetes/diabetes5.htm
Full transcript