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Sonic Vibration Affects The Metabolism of Yeast Cells Growin

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on 25 January 2016

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Transcript of Sonic Vibration Affects The Metabolism of Yeast Cells Growin

Sonic Vibration Affects The Metabolism of Yeast Cells Growing in Liquid Culture:
A Metabolomic Study

Material & Methods
Yeast (saccharomyces cerevisiae) Why?
Material & Methods
Previous Study
Plants respond to different music and high frequency sound waves . (Xiujuan et al. 2003)
Specific frequencies can stimulate intestinal contractions and duodenal passage of barium in humans (Polous and Kurko 1991)
Sonic waves induce contractive conformational changes of the mitochondrial transmembrane ATPase, affecting ATP production (Syroeshkin et al. 1998)

Using a metabolomic approach, it shows that audible frequency sound influences yeast cell metabolism
Different sound frequencies induce different metabolic and physiological responses in yeast cells
Raphael Bastos Mereschi Aggio * Victor Obolonkin * Silas Granato Villas-Boas
Metabolomics @ Springer (2012) Volume 8 pp 670–678 Impact Factor : 4.433
Audible sounds, consist of oscillating pressure waves transmitted through solids, liquids, or gases, that if sufficiently strong stimulate the mechanical of sensory cell
Music and sounds can affect human emotions and mood
Most study done only focused on brain and sensorimotor structures rather than cellular level
Alternative form of therapy to treat various illnesses
To date little is known about the effects of sonic waves on the cellular metabolism
To exclude the complexity of multicellular organisms and to eliminate the influence of nervous and auditory systems
The genome, biochemistry and metabolome are extremely well characterised, and as such provide a powerful model to test the role of sound on cell metabolism
Yeast was culture in shake-flasks & inoculated with an overnight grown pre-inoculum prepared in the same growth medium
Cultivations were performed using the same rotatory shaker and incubated in the same incubation room
The experimental set up as shown
Material & Methods
The yeast cultures were exposed to four different conditions
1) High frequency, 10 kHz, recording 89 dBA, sampled at 705 Kbps
2) Low frequency, 100 Hz, recording 92 dBA, sampled at 705 Kbps
3) Broad-band music, sampled at 320 Kbps and 80–92 dBA
4) ‘‘silence’’—control conditions
The sound stimuli were played continuously for the duration of the experiment
Result & Discussion
The yeast cells growing under sonic stimuli presented longer lag phase and shorter exponential growth when compared to cells growing in the silence.
All sonic stimuli tested not only increased the growth rate of the yeast cells by 12% but they also reduced biomass production by 14%.
Material & Methods
Full transcript