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Transcript of r
COMPOSITION OF ESSENTIAL OIL
FROM ARTEMISIA ABYSSINICA Investigation antioxidant activity of the plants. AIMS OF RESEARCH Analyzing the chemical composition of these oils by Gas chromatography- Mass spectroscopy hyphenated system. An antioxidant is a molecule that inhibits the oxidation of other molecules. Oxidation is a chemical reaction that transfers electrons or hydrogen from a substance to an oxidizing agent. Free radicals and Antioxidant An essential oil is a concentrated hydrophobic liquid containing volatile aroma compounds from plants volatile oils Therapeutically
Starting materials Uses The distinctive components in essential oils defend plants against insects, environmental conditions, and disease. Essential oil All official volatile oils are of vegetable origin. Normally pre-exist in the plant – stored in a special secretory tissue (e.g. Citrus peel oil cells or oil ducts in fruit. Group Group Member Member Member Member Member Member Member (cc) photo by theaucitron on Flickr (cc) photo by theaucitron on Flickr copy paste branches if you need more.... roots bark leafs flowers fruit # The aromatic-ring structure of essential oils is much more complex than the simpler, linear carbon-hydrogen structure of fatty oils. Essential oils also contain sulfur and nitrogen atoms that fatty oils do not have. Major Categories of Aromatic Oil Compounds TERPENOIDS PHENYLPROPANOIDS NON-TERPENOID ethers phenols Oxides aldehydes alcohols Aldehydes Phenols phenolic ketones Though, the essential oil composition of A. ABYSSINICA in Yemen has not been previously reported. The oils differ in their chemical compositions according to
geographic origin of the plants. The essential oil of A.ABYSSINICA from different regions of the world has been reported by many workers. Reported differrences in essential oil composition of A.ABYSSINICA collected at different places in Ethiopia Yemen and Iran finally we report for the first time the antioxidant of the essential oil of A.ABYSSINICA leaves from Yemen and the newly identified compounds Different molecules in the same essential oil can exert different effects. Other compounds in German chamomile perform still different functions, such as speeding the regeneration of tissue. The azulene in German chamomile has
powerful anti-inflammatory compounds. The bisobolol German chamomile has
sedative & mood-balancing properties. The chemical structure of an essential oil determines its function. Phenols generally create antibacterial activity. Carvacrols have anti-inflammatory activity Limonines are antiviral. The aerial parts of A. abyssinica were collected from Sana'a,Taiz, Alhodaida , were identified by Dr. Hassan Ibrahim, Department of Biology, Faculty of Science, Sana'a University. Voucher specimens were deposited in the Herbarium of Department of Pharmacognosy, Faculty of Pharmacy, Sana’a University. Methodology and Collection ARTEMISIA ABYSSINICA EXTRACTION Air-dried leaves (50 g) were subjected to hydrodistillation in all glass Clevenger type apparatus for 3 h in accordance with British Pharmacopoeia (1980) method. 50g Air-dried leaves 500ml Dist. H2O(1:10 Hydrodistillation for 3 h in all glass Clevenger type apparatus Clevenger Apparatus
Hydrodistillation process The essential oil was collected and dried over anhydrous sodium sulphate (Na2SO4) and stored at 4oC until analyzed. storage Identification of components Library Of National Institute Of Standard And Technology (NIST) Database Gas chromatography – mass spectrometric (GC-MS) analyses comparison of their mass spectra with published spectra [Adams, 2007 (cc) image by nuonsolarteam on Flickr The Results Plant % yield (v/w) (ml/100g) ARTEMISIA ABYSSINICA 5+7= (cc) image by anemoneprojectors on Flickr 1ml In the essential oil of A. abyssinica, 14 compounds were identified, representing 92.65% of the total oil. The most abundant components were: The percentage of essential oil composition of ARTEMISIA ABYSSINICA according to chemical classification one two (cc) image by anemoneprojectors on Flickr oxygenated sesquiterpenes oxygenated monoterpenes (28.37%) (51.30%) Chemical composition of ARTEMISIA ABYSSINICA -Pinene -Phellandrene -Terpinene Linalool Camphor Borneol 1-Terpinen-4-ol Bornyl acetate Cinnamyl ethyl ester 1.540 0.491 0.401 2.445 22.888 0.821 2.231 2.68 1.086 Nerolidol Davanone Chamazulene Palmitic acid Total 8.96 42.34 4.469 1.633 92.65 following Camphor (22.8%) Terpenes:
Oxygenated bicyclic monoterpene.
Acts as slight local anesthetic and antimicrobial substance Terpenes:
a flavoring agent and in perfumery. Nerolidol (8.96%) Davanone (42.3) Terpenes:
Antifungl , Antioxidant. Terpenes:
anti-inflammatory , antioxidant, cosmetic products. Chamazulene (4.46%) Terpenes:
uses Linalool (2.4%) Comparing our results with other data regarding the chemical composition of EOAb, it was found that the sesquiterpenes, davanone, nerolidol and chamazulene were not mentioned as a main components previously. It is possible that the analysed essential oil was from a new chemotype of A. abyssinica . and the study are showed the chemical composition in the studied oil note changed according to the ecological factors and the chemotype is genetically fixed. If the chemotype davanone of A. abyssinica is confirmed, the plant may be a good source of davanone oil. It is worth mentioning that davanone is also found as major compound in other Artemisia species.
Davanone (42.34%), as the main component in A. abyssinica oil, was found also in A. persica (60.56%), A. Khorasanica (36.4%), A. herba (8.49 %), collected from Iran), A. vulgaris var. indica (71.56 %).from Thailand
On the other hand, camphor (22.88%), as the second main compound in the oil, has been found at high concentrations in essential oils of some Artemisia species Discussions further study for activity and toxicity of extract oil. Recommendation Thanks for
prof. nasser Awadh Hydrocarbons :Stimulant, decongestant, antiviral, antitumour Compound Properties Alcohols : Antimicrobial, antiseptic, tonifying, spasmolytic Sesquiterpene alcohols: Anti-inflammatory, anti-allergenic Phenols Antimicrobial, irritant, immune stimulating Aldehydes Spasmolytic, sedative, antiviral Cyclic aldehydes Spasmolytic, Ketones :Mucolytic, cell-regenerating, neurotoxic Esters: Spasmolytic, sedative, antifungal Oxides: Expectorant, stimulant Coumarins: UV sensitising, antimicrobial Sesquiterpenes: Anti-inflammatory, antiviral Phenylpropanes: Carminative, anaesthetic Sesquiterpene Lactones: Mucolytic, immune stimulating Analyzing the chemical composition of these oils by Gc-ms chyphenated system. Isolation of Essential oils by Clevenger apparatus type. Diphenyl Picryl Hydrazyl (DPPH) radical scavenging method Investigation antioxidant activity of the plant. violet yellow Nowadays, there are many health problems which cannot treated easily or cannot be treated by either traditional or recent medical systems as; dangerous resistance infections, oxidation reactions (due to environmental pollution, canned foods, preservatives in different foods and drinks……etc.) which result in many organic diseases as diabetes mellitus, cardiovascular problems The analysed essential oil is a new chemotype of A. abyssinica. Conclusion Emad Abdallah Al -Hetar by: Life Elixir Group Ammar Mohamed Al Mathhaji Amro Ahmed Al Howbani Fahmi Derhim Abdaljalil Mohammed Ahmed Alhadad Mohammed Fuad Al Hakimi Yousif Farhan Al Sharabi Aims of the research Introduction family: Asteraceae habit: uses: sana,a Taiz Alhodiada 1.6ml .8 0.8ml essential oil (2S)-2-[(2S,5R)-5-ethenyl-5-methyloxolan-2-yl]-6-methylhept-5-en-3-one comparing betwen three samples ( sana'a Taiz, Alhodiada) Dr.Somaya Al Mathhaji And for all. free radical :are atoms, molecules, or ions with unpaired electrons or an open shell configuration. Free radicals may have positive, negative, or zero charge Linoleic acid 14 0.665 The chemotype is genetically fixed. The essential oil have antioxidant effect. The essiontial oil have antioxidant effect Antioxidant activity B