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Kloneren van eukaryoot DNA

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by

myrthe prent

on 30 November 2014

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Transcript of Kloneren van eukaryoot DNA

Invoegen van DNA in plasmide
Restricie enzymen
Blunt ends
Sticky ends
DNA ligase
Kloneren van eukaryoot DNA
3 stappen:
1: Invoegen van DNA in het plasmide

2: Inbrengen en vermenigvuldigen van de recombinante plasmiden in bacteriën

3: Selectie van de cellen die het recombinante DNA bezitten
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