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How Temperature Affects Moldy Bread

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Pryce Markson

on 7 November 2013

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Transcript of How Temperature Affects Moldy Bread

How Temperature Affects Moldy Bread
By Pryce Markson
Problem Statement
How does temperature affect mold growth on bread?
If bread is placed at room temperature, a refrigerator, and a freezer for 21 days, then the piece at room temperature will grow the most mold.
3 10x10 cm slices of whole wheat bread
3 ziploc bags
120 mL measuring cup
15 mL water 21C / room temperature
30 cm ruler
1. Leave 3 10x10 cm2 slices of whole wheat bread at room temperature, in a ziploc bag, for 24 hours.
2. Pour 5 mL of 21C water onto each slice.
3. Reseal each bag.
4. Place one bag in refrigerator, one bag in freezer, and leave one bag at room temperature in dark place.
5. Leave them there for 21 days.
6. Measure mold every day in mm2.
7. Plot the data on a graph.
Manipulated variable: Temperature
Responding variable: Amount of mold
Constants: Size of bread, amount of water, type of bread, bag
Mold Measurement
During the first seven days, none of the bread grew mold. The slice that was kept at room temperature grew 18 mm2 of mold by the end of the twenty-first day. The average amount of mold was 7.53 mm2 for the slice at 21C. Mold didn't start to grow until day 10 for the slice at 3.2C. At the end of the experiment, 7 mm2 grew on slice 2, at an average of 3.54 mm2. The slice at -18C grew 0 mm2 of mold for the entire length of the experiment.
My data supports my original hypothesis. Temperature does affect the amount of mold on bread. Also, my data showed that the speed of mold growth was affected by temperature. Originally, I hypothesized that the mold would grow after only a few days, but it took 8 days for it to grow. Next time, I will allot more time for the mold to grow and I can also use different types of bread to see if the type of bread will affect mold growth too.
The importance of this experiment is that the economy has hurt a lot of families and if they know the proper temperature to store their bread, it will last longer and people will save money. In business, the bread companies, trucking companies, and the grocery companies could also use this info to reduce spoilage.
The problem was to determine how temperature affects mold growth on bread. It was hypothesized that if bread is placed at room temperature, a refrigerator, and a freezer for 21 days, then the piece at room temperature will grow the most mold.
The procedure followed was taking 3 equal slices of bread, dropped 5 mL of water on each slice, and putting them each in a ziploc bag. One was placed at room temperature, one was in the refrigerator, and one was in the freezer. They were left there for 21 days, and checked every day. Then the data was plotted on a graph based on the measured amount of mold in mm2.
It was concluded that the bread at room temperature grew the most mold. The results of the experiment did support the hypothesis because the bread slice in the freezer did not grow any mold and the warmer the temperature was, the more mold grew.
World Wide Web: Food Molds- Mold on Foods <www.whatscookingamerica.net/Q-A/Mold.htm> 10/6/13

World Wide Web: Mold
<en.wikipedia.org/wiki/Mold#common_molds> 10/6/13

Head, Honor, Bread, Laguna Hills, CA, QEB Publishing, Inc., 2006, pp. 10-13
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