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jesus pineda

on 18 June 2013

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Transcript of Internship

Kary Mullis
En 1983 utilizó una técnica que consistía
en agregar 2 "primers" y una polimerasa
de ADN al medio de reacción.
La temparatura depende de varios
factores y es relativamente
específica para cada primer.
La longitud de los primers y la secuencia designan los parámetros de una amplificación, una fórmula simple para calcular la Tm (“melting temperature”) es la siguiente:
La temperatura se eleva a 72ºC
ya que es la temperatura a la que la
Taq polimerasa alcanza su máxima actividad.

Las cantidades óptimas de Taq polimerasa necesarias
para la síntesis de ADN están alrededor de 2 unidades
en 25 µl de volumen final de reacción.
Permite genotipar la especie que provoca un cuadro infeccioso.
Para chequeos rutinarios.
El diagnóstico de enfermedades hereditarias presentes en el genoma.
Paleontología, antropología y ciencias forenses.
Jesus Internship Presentation of learning (POL)

Signs and Symptoms

1. Loss of coordination (ataxia) in the arms and legs.

2. Fatigue, energy deprivation and muscle loss.

3. Vision impairment, hearing loss, and slurred speech.

4. Aggressive scoliosis a serious heart condition.

5. Recessive inherited disease that causes progressive damage to the nervous system.
At first.......
Study Friedreich’s ataxia
Knockout Mice
(FXN) Frataxin gene
Role and project during

1. Visual
2. Detailed
3. Easy to understand

Design a protocol for the (PCR) machine

3 main components
that where incorporated
Protocol Pages
Skill sets coming into internship

-Computer skills- Photoshop, Illustrator
(useful for protocol).
-Adapting to different work settings.
(From lab to computer)
-Applying creativity to work products

Skill sets accrued during internship.
-Advocate for myself.
-Lab procedures.
- More hands-on experiments.
-Understanding different aspects
of (PCR) and Electrophoresis gel.
-Overall the experience of working in a lab

Importance of Communication
-Experience during (PCR) process-lab.
-Important in the future.
-New mindset.
-Advocating for yourself is essential
for a great working environment.

Consider Future High
School Student interns:

-Integrate our schools principles of getting hands- on experience.
-Lab culture
-Great learning experience and environment
-Early experience can help a high school student in pursuing a career within research

Experience in the
professional working world.
-Great experience.
-Idea for future careers.
-A new perspective in research
-It just gets harder, so work harder!

Special Thanks To........
Mike Lovci
Jason Nathanson
Internship Mentors
PCR and Electrophoresis Gel
- Followed Crimson Taq
-DNA Polymerase Protocol
Followed protocol-Step by step
- Isolate SNP that influences the taste of cilantro(taste like soap)

- Present this to the younger students
-Continue developing the hardware
-Start working on protocol for second
-Run (PCR) on current.

2% gel-
-75µL of 1X TBE
-1.5grams of agarose
-Boil in microwave
-Added Syber Safe
-Waited to cool of
-Ran Electrophoresis
-5µL = ladder.
-SP results
-My results
Same bands
of DNA
-Shows how much replication
-Replicated the same.

1st PCR Test
Same DNA

X4 + 10 % into a
Master tube
To then be separated to
PCR tubes

Two 5μµL ladders
`1 hour @100v
3 different samples in the same range
NTC-No genetic material
Sanford Consortium for Regenerative Medicine
Junior Reflection....


~Senior Year


~Future with Internship
Full transcript