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Test for Vitamin-C using Iodine Titration

Chem 111 final project

Sam Martin

on 10 December 2012

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Transcript of Test for Vitamin-C using Iodine Titration

Why? As one of the essential vitamins for sustained human life knowing how much vitamin-C someone take in is necessary to maintain a healthy life style. What's happening? How? Results Procedures Redox Titration Sam Martin
Jesse Young TsaiWei Cheng Testing for Vitamin-C
Using Redox Titration What it means for you Preparation Standardization Final Testing Ascorbic acid+I^2 2I^-+dehydrascorbic acid Too Much = Acidic Qualities Damage Teeth Too Little = Vitamin-C Deficiency 1% Starch Indicator I^2 Solution Boil water
Add 100.0ml of boiling water to .50g of starch
Dissolve starch mix 0.250g of potassium iodide and 0.134g of potassium iodate in 100.0ml of deionized water
Add 15.0ml of sulfuric acid
Dilute to 250.0ml Known Ascorbic Acid Solution Dissolve0.251g in 100.0ml of deionized water
Dilute to 250ml Testing Prepare 25.0ml samples of solution
Add 10 drops of starch indicator
Perform Titrations Found Molarity of I^2 solution 8.5436E-2±2.7440E-2M Prep of Juices Add 25ml of juice sample to 100.0ml of water
Add 1.0ml of starch solution
Add 5.0ml of 1M hydrochloric acid Test Divide prepared amounts in half equally
Titrate the samples + Math = Pomegranate 3.060E-3g±1.199E-4g
Orange Juice 0.04996g±0.003883g
Sobe Water 0.04514g±0.002673g
Emergen-C 0.7992g±.01736g Per 12.5ml of juice If you are borderline for Vitamin-C Deficiency then Emergen-C is a juice to increase your levels However, if you have a healthy level already then drinking Emergen-C could just be an over exposure of acid to your teeth Fun Facts: Also known as Ascorbic Acid


Helps in healing

Min intake of 10mg daily

Vitamin-C deficiency is also know as Scurvy and can be fatal Keep in mind 1 US cup = 236.588ml Multiplication factor of 18.927 Amounts per 1 Cup Orange juice = 0.8543g Sobe Water = 0.9456g Pomegranate = 0.05792g Emergen-C = 15.1264g Equipment 10ml
Graduated Cylinders 4x125ml Erlenmeyer Flasks Buret Stand
Buret holder
25ml buret Hot plate 3x50ml
3x400ml Beakers Glass Stiring rod
Scopula Analitical Balance Reagents Deionized water Potassium Iodide Potassium Iodate 3M Sulfuric Acid 1M Hydrochloric Acid Ascorbic Acid Soluble Starch Pomagranate Juice Orange Juice Emergen-C Sobe Water Recommended not to exceed 2000mg daily Low overdose risk but can cause tooth damage, kidney stones, and possible change in Stomach PH Sources Too Little = Scurvy AMINUDDIN, M., FAIYAZ HUSSAIN MADANI VAID, and KARAMAT MEHMOOD. "STATISTICAL EVALUATION OF THE N-BROMOSUCCINIMIDE AND IODINE AS TITRIMETRIC METHODS FOR ESTIMATION OF VITAMIN C IN ORANGE JUICE AND PHARMACEUTICAL PREPARATIONS." Pakistan Journal of Pharmaceutical Sciences 16.2 (2003): 69-76.

Bergström, Therese, Jan Bergman, and Lennart Möller. "Vitamin A And C Compounds Permitted In Supplements Differ In Their Abilities To Affect Cell Viability, DNA And The DNA Nucleoside Deoxyguanosine." Mutagenesis 26.6 (2011): 735-744. Environment Complete. Web. 9 Dec. 2012.

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"Determination of Vitamin C by Redox Titration with Iodate." University of Canterbury Outreach. Ed. University of Canterbury. University of Canterbury, n.d. Web. 19 Nov. 2012. 

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GUEBEANT,, N., A. RASMUSSEN, and R. DUTCHES. "THE VALUE OF THE CHEMICAL TITRATION METHOD IN DETERMINING THE VITAMIN C POTENCY OF CERTAIN FOOD SUBSTANCES." The Journal of Nutrition. N.p.: Department of Agriculture and Biological Chemistry at Pennsylvania State University, n.d. 667-75. 14 Dec. 1934. Web. 8 Dec. 2012.

Institute of Medicine. Food and Nutrition Board. Dietary Reference Intakes for Vitamin C, Vitamin E, Selenium, and Carotenoids. National Academy Press, Washington, DC, 2000.

Michael M Aruldhas, et al. "Ameliorative Effect Of Vitamin C On Hexavalent Chromium-Induced Delay In Sexual Maturation And Oxidative Stress In Developing Wistar Rat Ovary And Uterus." Toxicology & Industrial Health 28.8 (2012): 720-733. Environment Complete. Web. 9 Dec. 2012.

Sarubin Fragaakis A, Thomson C. The Health Professional's Guide to Popular Dietary Supplements. 3rd ed. Chicago, Il: American Dietetic Association; 2007.

"Vitamin C (Ascorbic Acid): Antioxidant Used for Colds and Other Health Conditions." WebMD. WebMD, n.d. Web. 09 Dec. 2012.

Vitamin C. Office of Dietary Supplements. http://ods.od.nih.gov/factsheets/VitaminC-HealthProfessional. Accessed December 8th, 2012.

"Vitamin C." QuickFacts. Office of Dietary Supplements, n.d. Web. 09 Dec. 2012.

Zehra, Seemab, and Mukhtar Khan. "Dietary Vitamin C Requirement of Fingerling, Cirrhinus Mrigala (Hamilton), Based on Growth, Feed Conversion, Protein Retention, Hematological Indices, and Liver Vitamin C Concentration." Journal of the World Aquaculture Society (2012): n. pag. Print.

Zeratsky, Katherine, R.D., L.D. "Is It Possible to Take Too Much Vitamin C?" Mayo Clinic. Mayo Foundation for Medical Education and Research, 20 Mar. 2012. Web. 09 Dec. 2012. ml ml ml ml ml ml ml ml ml ml ml ml Easy to measure Reactions occur in equal ratios First reaction: Once all the Ascorbic acid is used up the I^2 the reacts then reacts with starch to turn the solution blue Two reactions occur Second Reaction:
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