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1.3.1 DNA Detectives

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by

Lori Richardson

on 20 September 2017

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Transcript of 1.3.1 DNA Detectives

Restriction Enzymes
Restriction enzymes are a tool that allows us to pinpoint human identity down to single differences in our DNA.

Work through the following simulation so you can see these molecular scissors in action.
What Are
Restriction Enzymes
Naming
Restriction Enzymes
Hin
1.3.1 DNA Detectives
Gel Electrophoresis of DNA
Making the Cut
Like all enzymes, restriction enzymes are highly specific. They cut DNA only within very precise recognition sequences.

This cutting in a unique pattern is very useful to the science of medicine.
Cutting with the Enzyme EcoRI
EcoRI looks for the sequence GAATTC
Cutting with the Enzyme PstI
PstI looks for the sequence CTGCAG
Review How
Restriction Enzymes Cut

Cold Springs Harbor Laboratory
Dolan Learning Center

https://www.dnalc.org/resources/animations/restriction.html
R.E.
M
M
M
M
Virus
R.E.
R.E.
Viral DNA
M = Methyl Groups
III
Genus: Haemophilus influenzae
Strain: D
Order of Identification
Naming
Restriction Enzymes
Eco
R
I
Genus: Escherichia coli
Strain: RY13
Order of Identification
d
CTGCAG
GACGTC
Palindrome
CTGCA
G
G
ACGTC
Sticky Ends
GAATTC
CTTAAG
Palindrome
AATTC
G
G
CTTAA
Sticky Ends
Cutting with the Enzyme HindIII
HindIII looks for the sequence AAGCTT
AAGCTT
TTCGAA
Palindrome
AGCTT
A
A
TTCGA
Sticky Ends
Gel Electrophoresis of Restriction Fragments
Gel Basics
Genetic Science Learning Center

Gel Electrophoresis Virtual Lab
DNA Interactive

Gel Electrophoresis
http://learn.genetics.utah.edu/content/labs/gel/
http://www.dnai.org/text/mediashowcase/index2.html?id=1014
Pairing of Bases
G T T G G G A A
A T T C A A C C C
1
2
3
4
5
6
*
Person 1
G G A A T T A A G C T T A T T G A A T T C T T A T A G A A T T C G GG G C C C A A G C T T A T G A A T T C A A T T
C C T T A A T T C G A A T A A C T T A A G A A T A T C T T A A G C C C C G G G T T C G A A T A C T T A A G T T A A
Person 2
C C A T A T A G A A T T C A A G C T T A A G A A T T C G G G G G A A C G T T G A A T T C A A T T A A T T G G G
G G T A T A T C T T A A G T T C G A A T T C T T A A G C C C C C T T G C A A C T T A A G T T A A T T A A C C C
1
2
3
4
1
2
3
4
16
7
17
5
8
10
13
12
Before
We Start

Humans Share
of Their DNA
99
+%
How do we sequence the human genome?
So how can we tell you apart from everyone else in the classroom, school, or even the world!
Full transcript